The isolation and characterization of XC3H-3b: a CCCH zinc-finger protein required for pronephros development

Biochem Biophys Res Commun. 2003 Aug 29;308(3):566-72. doi: 10.1016/s0006-291x(03)01419-0.


We describe the isolation and characterization of the RNA-binding protein XC3H-3b that is expressed during pronephros development. XC3H-3b is a member of the TTP/TIS family of CCCH tandem zinc-finger proteins, which are physiological stimulators of instability for the mRNA encoding tumor necrosis factor-alpha in certain cell types. XC3H-3b is localized primarily to the mesodermal tissues around the pronephros. Overexpression of XC3H-3b markedly and specifically inhibits kidney development. Morpholino-mediated knockdown of XC3H-3b also results in defects in nephrogenesis. In both cases, the expression of numerous pronephric marker genes, such as Xlim-1, Xpax-2, Xpax-8, Xwnt-4, and XWT1, is decreased and morphological development of the pronephric tubules is abrogated. We conclude that XC3H-3b plays an important role in the regulation of pronephros differentiation. This is the first report of a gene localized around the pronephros that regulates pronephros development.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Kidney / growth & development*
  • Kidney / metabolism
  • Kinetics
  • Molecular Sequence Data
  • Oligonucleotides, Antisense / genetics
  • RNA-Binding Proteins / chemistry
  • RNA-Binding Proteins / genetics*
  • RNA-Binding Proteins / physiology*
  • Sequence Alignment
  • Xenopus Proteins / chemistry
  • Xenopus Proteins / genetics
  • Xenopus Proteins / physiology
  • Xenopus laevis / anatomy & histology
  • Xenopus laevis / growth & development*
  • Xenopus laevis / metabolism
  • Zinc Fingers


  • Oligonucleotides, Antisense
  • RNA-Binding Proteins
  • Xenopus Proteins
  • ZFP36L2 protein, Xenopus