Mechanisms and enzymes involved in SARS coronavirus genome expression

J Gen Virol. 2003 Sep;84(Pt 9):2305-2315. doi: 10.1099/vir.0.19424-0.

Abstract

A novel coronavirus is the causative agent of the current epidemic of severe acute respiratory syndrome (SARS). Coronaviruses are exceptionally large RNA viruses and employ complex regulatory mechanisms to express their genomes. Here, we determined the sequence of SARS coronavirus (SARS-CoV), isolate Frankfurt 1, and characterized key RNA elements and protein functions involved in viral genome expression. Important regulatory mechanisms, such as the (discontinuous) synthesis of eight subgenomic mRNAs, ribosomal frameshifting and post-translational proteolytic processing, were addressed. Activities of three SARS coronavirus enzymes, the helicase and two cysteine proteinases, which are known to be critically involved in replication, transcription and/or post-translational polyprotein processing, were characterized. The availability of recombinant forms of key replicative enzymes of SARS coronavirus should pave the way for high-throughput screening approaches to identify candidate inhibitors in compound libraries.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Catalytic Domain
  • Coronavirus 3C Proteases
  • Coronavirus Papain-Like Proteases
  • Cysteine Endopeptidases / genetics
  • Cysteine Endopeptidases / metabolism
  • Frameshifting, Ribosomal
  • Gene Expression Regulation, Viral*
  • Genome, Viral*
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Papain / genetics
  • Papain / metabolism
  • Protein Biosynthesis
  • RNA Helicases / biosynthesis
  • RNA Helicases / genetics
  • RNA, Messenger / chemistry
  • RNA, Messenger / genetics
  • SARS Virus / enzymology
  • SARS Virus / genetics*
  • SARS Virus / isolation & purification
  • Sequence Alignment
  • Viral Proteins / genetics*
  • Viral Proteins / metabolism

Substances

  • RNA, Messenger
  • Viral Proteins
  • Cysteine Endopeptidases
  • Coronavirus Papain-Like Proteases
  • Papain
  • papain-like protease, SARS coronavirus
  • Coronavirus 3C Proteases
  • RNA Helicases

Associated data

  • GENBANK/AY291315