[D-limonene induces apoptosis of gastric cancer cells]

Zhonghua Zhong Liu Za Zhi. 2003 Jul;25(4):325-7.
[Article in Chinese]


Objective: To investigate the apoptosis effect induced by D-limonene on BGC-823 gastric cancer cells.

Methods: The expression of p53, bc1-2 in BGC-823 cells and qualitative, quantitative index of cell apoptosis were detected with MTT, electron microscopy, flow cytometry and immunohistochemical method.

Results: D-limonene could induce the formation of apoptotic bodies in a dose- and time-dependent manner. The expression of bcl-2 protein decreased and p53 protein increased in BGC-823 cells treated with D-limonene, compared with the control cells.

Conclusion: D-limonene exerts its cytotoxic effect on BGC-823 gastric cancer cells by inducing apoptosis.

MeSH terms

  • Adenocarcinoma / metabolism
  • Adenocarcinoma / pathology*
  • Antineoplastic Agents, Phytogenic / administration & dosage
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Apoptosis / drug effects*
  • Cell Line, Tumor
  • Cyclohexenes
  • Dose-Response Relationship, Drug
  • Humans
  • Limonene
  • Proto-Oncogene Proteins c-bcl-2 / metabolism
  • Stomach Neoplasms / metabolism
  • Stomach Neoplasms / pathology*
  • Terpenes / administration & dosage
  • Terpenes / pharmacology*
  • Tumor Suppressor Protein p53 / metabolism


  • Antineoplastic Agents, Phytogenic
  • Cyclohexenes
  • Proto-Oncogene Proteins c-bcl-2
  • Terpenes
  • Tumor Suppressor Protein p53
  • Limonene