Early hyperbaric oxygen therapy attenuates disease severity in lupus-prone autoimmune (NZB x NZW) F1 mice

Clin Immunol. 2003 Aug;108(2):103-10. doi: 10.1016/s1521-6616(03)00091-3.


The effects of hyperbaric oxygen (HBO(2)) therapy on the immune system are reported including potential changes to the CD4/CD8 ratio and a decreased proliferation of lymphocytes during exposure. The immunosuppressive effect of HBO(2) had been suggested to be applicable for the treatment of certain autoimmune diseases. (NZB x NZW) F1 hybrid mice, the unique lupus-prone mice, have been used for elucidating the pathogenesis of SLE. To investigate the effect of HBO(2) on NZB/W F1 lupus-prone mice, 32 female mice were divided into four groups. Three groups of mice were treated with HBO(2) (2.5 atm abs (ATA) for 90 min daily over 2 weeks) starting at (A) 3 months, (B) 6 months, or (C) 8 months of age, while the remaining group (D) served as control. Animals were followed until 11 months of age. Experimental parameters included life span, proteinuria, peripheral lymphocytes, anti-dsDNA antibody titers, and renal histopathology. HBO(2) treatment resulted in increased survival, decreased proteinuria, alterations in lymphocyte-subset redistribution, reduced anti-dsDNA antibody titers, and amelioration of immune-complex deposition in groups A and B. Our data demonstrated that HBO(2) therapy attenuated disease severity in NZB/W F1 mice. HBO(2) treatment may be of use in the clinical treatment of lupus patients and would benefit from further study.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Age Factors
  • Animals
  • Antibodies / blood
  • DNA, Single-Stranded / blood
  • DNA, Single-Stranded / immunology
  • Disease Models, Animal
  • Female
  • Flow Cytometry
  • Hyperbaric Oxygenation*
  • Immunoglobulin G / blood
  • Immunoglobulin M / blood
  • Kidney / immunology
  • Kidney / pathology
  • Lupus Erythematosus, Systemic / immunology
  • Lupus Erythematosus, Systemic / pathology
  • Lupus Erythematosus, Systemic / prevention & control*
  • Lymphocyte Subsets / immunology
  • Mice
  • Mice, Inbred NZB
  • Proteinuria / pathology
  • Proteinuria / prevention & control
  • Spleen / cytology
  • Spleen / immunology
  • Survival Rate


  • Antibodies
  • DNA, Single-Stranded
  • Immunoglobulin G
  • Immunoglobulin M