Honokiol and magnolol induce Ca2+ mobilization in rat cortical neurons and human neuroblastoma SH-SY5Y cells

Eur J Pharmacol. 2003 Aug 8;474(2-3):199-204. doi: 10.1016/s0014-2999(03)02075-2.


We examined the intracellular Ca(2+) response in primary cultured rat cortical neurons and human neuroblastoma SH-SY5Y cells by Fluo 3 fluorescence imaging analysis. In these two kinds of neuronal cells, honokiol and magnolol increased cytoplasmic free Ca(2+) with a characteristic lag phase. The cytoplasmic free Ca(2+) increase was independent of extracellular Ca(2+), but dependent on activation of phospholipase C and inositol 1,4,5-triphosphate (IP(3)) receptors. These results suggest that honokiol and magnolol increase cytoplasmic free Ca(2+) through a phospholipase C-mediated pathway, and that the release of Ca(2+) from intracellular stores mainly contributes to the increase in cytoplasmic free Ca(2+). Thus, honokiol and magnolol may be involved in a new activation mechanism closely associated with intracellular Ca(2+) mobilization.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biphenyl Compounds / pharmacology*
  • Calcium / metabolism*
  • Cell Line, Tumor
  • Cells, Cultured
  • Cerebral Cortex / drug effects*
  • Cerebral Cortex / metabolism
  • Female
  • Humans
  • Lignans*
  • Neuroblastoma / metabolism*
  • Neurons / drug effects*
  • Neurons / metabolism
  • Pregnancy
  • Rats
  • Rats, Sprague-Dawley


  • Biphenyl Compounds
  • Lignans
  • magnolol
  • honokiol
  • Calcium