A linkage map of meadow fescue ( Festuca pratensis Huds.) and comparative mapping with other Poaceae species

Theor Appl Genet. 2003 Dec;108(1):25-40. doi: 10.1007/s00122-003-1399-5. Epub 2003 Aug 16.

Abstract

A genetic linkage map has been constructed for meadow fescue ( Festuca pratensis Huds.) (2n=2 x=14) using a full-sib family of a cross between a genotype from a Norwegian population (HF2) and a genotype from a Yugoslavian cultivar (B14). The two-way pseudo-testcross procedure has been used to develop separate maps for each parent, as well as a combined map. A total number of 550 loci have been mapped using homologous and heterologous RFLPs, AFLPs, isozymes and SSRs. The combined map consists of 466 markers, has a total length of 658.8 cM with an average marker density of 1.4 cM/marker. A high degree of orthology and colinearity was observed between meadow fescue and the Triticeae genome(s) for all linkage groups, and the individual linkage groups were designated 1F-7F in accordance with the orthologous Triticeae chromosomes. As expected, the meadow fescue linkage groups were highly orthologous and co-linear with Lolium, and with oat, maize and sorghum, generally in the same manner as the Triticeae chromosomes. It was shown that the evolutionary 4AL/5AL translocation, which characterises some of the Triticeae species, is not present in the meadow fescue genome. A putative insertion of a segment orthologous to Triticeae 2 at the top of 6F, similar to the rearrangement found in the wheat B and the rye R genome, was also observed. In addition, chromosome 4F is completely orthologous to rice chromosome 3 in contrast to the Triticeae where this rice chromosome is distributed over homoeologous group 4 and 5 chromosomes. The meadow fescue genome thus has a more ancestral configuration than any of the Triticeae genomes. The extended meadow fescue map reported here provides the opportunity for beneficial cross-species transfer of genetic knowledge, particularly from the complete genome sequence of rice.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Mapping*
  • Chromosomes, Plant
  • Crosses, Genetic
  • DNA, Plant / genetics*
  • DNA, Plant / isolation & purification
  • Edible Grain / genetics
  • Evolution, Molecular
  • Festuca / genetics*
  • Gene Rearrangement
  • Genetic Linkage*
  • Genetic Markers
  • Genome, Plant*
  • Genotype
  • Polymorphism, Restriction Fragment Length
  • Repetitive Sequences, Nucleic Acid
  • Species Specificity
  • Translocation, Genetic

Substances

  • DNA, Plant
  • Genetic Markers