Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2003 Sep 5;308(4):784-92.
doi: 10.1016/s0006-291x(03)01475-x.

NKIAMRE, a Novel Conserved CDC2-related Kinase With Features of Both Mitogen-Activated Protein Kinases and Cyclin-Dependent Kinases

Affiliations

NKIAMRE, a Novel Conserved CDC2-related Kinase With Features of Both Mitogen-Activated Protein Kinases and Cyclin-Dependent Kinases

Karen W L Yee et al. Biochem Biophys Res Commun. .

Abstract

We report the cloning of the NKIAMRE gene located on human chromosome 5q31.1. It encodes a novel 52kDa Cdc2-related kinase with a 1.5kb open reading frame. Like MAP kinases, NKIAMRE contains a Thr-X-Tyr (TXY) motif in the activation loop domain. Similar to cdks, NKIAMRE contains the putative negative regulatory Ser14 and Tyr15 residues and the cyclin-binding motif, NKIAMRE, from which it derives its name. Human NKIAMRE has significant amino acid identity to related kinases in rat, mouse, Caenorhabditis elegans, and Drosophila, and is widely expressed in human tissues and cell lines. Confocal microscopy demonstrates that NKIAMRE localizes to the cytoplasm. NKIAMRE is activated by treatment of cells with phorbol 12-myristate 13-acetate. Mutation of the ATP-binding Lys-33 to arginine and the Thr-Glu-Tyr motif to Ala-Glu-Phe abolished its ability to phosphorylate myelin basic protein. NKIAMRE is a member of a conserved family of kinases with homology to both MAP kinases and cyclin-dependent kinases.

Similar articles

See all similar articles

Cited by 9 articles

See all "Cited by" articles

MeSH terms

LinkOut - more resources

Feedback