Detection of a metallo-beta-lactamase (IMP-1) by fluorescent probes having dansyl and thiol groups

Org Biomol Chem. 2003 Jan 7;1(1):17-20. doi: 10.1039/b209086d.

Abstract

A fluorescent probe for the detection of a metallo-beta-lactamase (IMP-1), N-[2-(5-dimethylaminonaphthalen-1-ylsulfonylamino)ethyl]-3- mercaptopropionamide (Dansyl-C2SH), 1, was designed based on combining the inhibitory function of mercaptocarboxylate and a fluorophore. The binding of 1 to IMP-1 was investigated by fluorescence spectroscopy. Compound 1 can act as fluorescent probe for detecting IMP-1 selectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carboxylic Acids / chemistry
  • Dose-Response Relationship, Drug
  • Fluorescent Dyes / pharmacology
  • Inhibitory Concentration 50
  • Models, Chemical
  • Spectrometry, Fluorescence
  • beta-Lactamases / analysis*
  • beta-Lactamases / chemistry*

Substances

  • Carboxylic Acids
  • Fluorescent Dyes
  • beta-lactamase IMP-1
  • beta-Lactamases