IL-17 enhances the susceptibility of U-2 OS osteosarcoma cells to NK cell lysis

Clin Exp Immunol. 2003 Sep;133(3):344-9. doi: 10.1046/j.1365-2249.2003.02234.x.

Abstract

We investigated the effect of the proinflammatory cytokine interleukin 17 (IL-17) on the lysis of osteosarcoma cells by human NK cells. NK cells and U-2 OS, MG-63, HOS osteosarcoma cell lines express the IL-17 receptor, the highest amount being found on U-2 OS. Pre-incubation of NK cells with IL-17 did not affect the cytotoxicity against osteosarcomas, that was increased when U-2 OS were pre-incubated with IL-17. In IL-17 treated U-2 OS osteosarcoma cells FACS analysis demonstrated an increased expression of fibronectin among the panel of adhesion molecules assayed, and the treatment with anti-fibronectin antibodies decreased the NK cytotoxicity. The comparison between interferon gamma (IFN-gamma) treated and IFN-gamma/IL-17-treated U-2 OS showed a decreased susceptibility to NK lysis associated with a reduced expression of CD49f on U-2 OS treated with IFN-gamma/IL-17. IL-17 appears to be a modulator of NK adhesion molecules on U-2 OS cells but antagonizes with IFN-gamma on NK lysis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Bone Neoplasms / immunology*
  • Bone Neoplasms / metabolism
  • Cytotoxicity Tests, Immunologic
  • Fibronectins / metabolism
  • Flow Cytometry
  • Humans
  • Integrin alpha2 / metabolism
  • Integrin alpha6 / metabolism
  • Interferon-gamma / therapeutic use
  • Interleukin-17 / therapeutic use*
  • Killer Cells, Natural / immunology*
  • Osteosarcoma / immunology*
  • Osteosarcoma / metabolism
  • Receptors, Interleukin / analysis
  • Receptors, Interleukin-17
  • Recombinant Proteins / analysis
  • Stimulation, Chemical
  • Tumor Cells, Cultured

Substances

  • Fibronectins
  • IL17RA protein, human
  • Integrin alpha2
  • Integrin alpha6
  • Interleukin-17
  • Receptors, Interleukin
  • Receptors, Interleukin-17
  • Recombinant Proteins
  • Interferon-gamma