MIP-T3 Associates With IL-13Ralpha1 and Suppresses STAT6 Activation in Response to IL-13 Stimulation

FEBS Lett. 2003 Aug 28;550(1-3):139-43. doi: 10.1016/s0014-5793(03)00860-3.

Abstract

To unravel the mechanism of interleukin-13 (IL-13)-specific functions, we sought to identify IL-13 receptor (IL-13R) binding molecules. A novel human IL-13Ralpha1 binding protein (IL13RBP1) has been identified using yeast tri-hybrid system, which was found to encode the same protein as MIP-T3 (microtubule interacting protein that associates with tumor necrosis factor (TNF) receptor associating factor-3 (TRAF3)). It constitutively associates with IL-13Ralpha1 and suppresses IL-4/13-induced signal transducer and activator of transcription-6 (STAT6) phosphorylation. IL-13-induced STAT6 activation was also inhibited as determined by dual luciferase assay and electrophoretic mobility shift assay (EMSA). These results suggest that MIP-T3 is a novel inhibitor of IL-13 signaling and may be a useful molecule in ameliorating various conditions in which IL-13 plays a central role.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Cloning, Molecular / methods
  • DNA / metabolism
  • Humans
  • Interleukin-13 / metabolism
  • Interleukin-13 / pharmacology*
  • Interleukin-13 Receptor alpha1 Subunit
  • Interleukin-4 / pharmacology
  • Mammals
  • Microtubule-Associated Proteins / drug effects
  • Microtubule-Associated Proteins / genetics
  • Microtubule-Associated Proteins / metabolism*
  • Microtubules / metabolism
  • Molecular Sequence Data
  • Phosphorylation / drug effects
  • Protein Interaction Mapping / methods
  • Proteins / metabolism
  • Receptors, Interleukin / drug effects
  • Receptors, Interleukin / genetics
  • Receptors, Interleukin / metabolism*
  • Receptors, Interleukin-13
  • STAT6 Transcription Factor
  • Signal Transduction
  • TNF Receptor-Associated Factor 3
  • Trans-Activators / drug effects
  • Trans-Activators / genetics
  • Trans-Activators / metabolism*
  • Transcription, Genetic
  • Two-Hybrid System Techniques
  • Tyrosine / metabolism

Substances

  • IL13RA1 protein, human
  • Interleukin-13
  • Interleukin-13 Receptor alpha1 Subunit
  • Microtubule-Associated Proteins
  • Proteins
  • Receptors, Interleukin
  • Receptors, Interleukin-13
  • STAT6 Transcription Factor
  • STAT6 protein, human
  • TNF Receptor-Associated Factor 3
  • TRAF3IP1 protein, human
  • Trans-Activators
  • Interleukin-4
  • Tyrosine
  • DNA

Associated data

  • GENBANK/AF242456