New members of the tomato ERF family show specific expression pattern and diverse DNA-binding capacity to the GCC box element

FEBS Lett. 2003 Aug 28;550(1-3):149-54. doi: 10.1016/s0014-5793(03)00757-9.


Four new members of the ERF (ethylene-response factor) family of plant-specific DNA-binding (GCC box) factors were isolated from tomato fruit (LeERF1-4). Phylogenetic analysis indicated that LeERF2 belongs to a new ERF class, characterized by a conserved N-terminal signature sequence. Expression patterns and cis/trans binding affinities differed between the LeERFs. Combining experimental data and modeled three-dimensional analysis, it was shown that binding affinity of the LeERFs was affected by both the variation of nucleotides surrounding the DNA cis-element sequence and the nature of critical amino acid residues within the ERF domain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis Proteins / genetics
  • Base Sequence
  • Binding Sites
  • DNA, Plant / metabolism*
  • DNA-Binding Proteins / chemistry
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism
  • Ethylenes / pharmacology
  • Gene Expression Regulation, Plant / drug effects
  • Lycopersicon esculentum / drug effects
  • Lycopersicon esculentum / genetics*
  • Lycopersicon esculentum / physiology
  • Models, Molecular
  • Molecular Sequence Data
  • Multigene Family
  • Nuclear Proteins / genetics
  • Organ Specificity
  • Plant Proteins / chemistry
  • Plant Proteins / genetics*
  • Plant Proteins / metabolism*
  • Protein Conformation
  • Regulatory Sequences, Nucleic Acid*
  • Sequence Analysis, DNA
  • Transcription Factors


  • Arabidopsis Proteins
  • DNA, Plant
  • DNA-Binding Proteins
  • Ethylenes
  • Nuclear Proteins
  • Plant Proteins
  • Transcription Factors
  • ethylene-responsive element binding protein
  • ethylene