Effects of 16S rRNA gene mutations on tetracycline resistance in Helicobacter pylori

Antimicrob Agents Chemother. 2003 Sep;47(9):2984-6. doi: 10.1128/AAC.47.9.2984-2986.2003.

Abstract

The triple-base-pair 16S rDNA mutation AGA(926-928)-->TTC mediates high-level tetracycline resistance in Helicobacter pylori. In contrast, single- and double-base-pair mutations mediated only low-level tetracycline resistance and decreased growth rates in the presence of tetracycline, explaining the preference for the TTC mutation in tetracycline-resistant H. pylori isolates.

MeSH terms

  • Cloning, Molecular
  • DNA Primers
  • Helicobacter pylori / drug effects*
  • Helicobacter pylori / genetics*
  • Helicobacter pylori / growth & development
  • Mutagenesis, Site-Directed
  • Mutation / genetics*
  • Mutation / physiology*
  • RNA, Ribosomal, 16S / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tetracycline Resistance / genetics*
  • Tetracycline Resistance / physiology*

Substances

  • DNA Primers
  • RNA, Ribosomal, 16S