Abstract
Beta-secretase (BACE) and glycogen synthase kinase (GSK 3) are two enzymes thought to play a role in Alzheimer's disease. We extracted mRNA from 90 Alzheimer and 81 control brains. Levels of mRNA were quantified for BACE and GSK 3 with TaqMan real-time RT-PCR. We found no change in the Alzheimer's disease brains relative to controls for either the BACE or the GSK 3alpha mRNA levels.
MeSH terms
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Alzheimer Disease / enzymology*
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Alzheimer Disease / genetics
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Alzheimer Disease / metabolism
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Amyloid Precursor Protein Secretases
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Amyloid beta-Protein Precursor / analysis
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Amyloid beta-Protein Precursor / genetics
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Aspartic Acid Endopeptidases
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Endopeptidases / genetics
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Endopeptidases / metabolism*
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Female
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Glial Fibrillary Acidic Protein / analysis
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Glial Fibrillary Acidic Protein / genetics
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Glycogen Synthase Kinase 3 / genetics
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Glycogen Synthase Kinase 3 / metabolism*
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Humans
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Male
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Microtubule-Associated Proteins / analysis
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Microtubule-Associated Proteins / genetics
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Phosphopyruvate Hydratase / analysis
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Phosphopyruvate Hydratase / genetics
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RNA, Messenger / analysis
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RNA, Messenger / biosynthesis
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Sex Characteristics
Substances
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Amyloid beta-Protein Precursor
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Glial Fibrillary Acidic Protein
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Microtubule-Associated Proteins
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RNA, Messenger
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Glycogen Synthase Kinase 3
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Amyloid Precursor Protein Secretases
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Endopeptidases
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Aspartic Acid Endopeptidases
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BACE1 protein, human
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Phosphopyruvate Hydratase