Analysis of ellagitannins and conjugates of ellagic acid and quercetin in raspberry fruits by LC-MSn

Phytochemistry. 2003 Sep;64(2):617-24. doi: 10.1016/s0031-9422(03)00281-4.


The use of gradient reversed phase HPLC with diode array and MS(n) detection for the analysis of ellagitannins, ellagic acid conjugates and quercetin conjugates in raspberries (Rubus idaeus L.) is described. MS(n) is a particularly powerful tool for the analysis of trace levels of natural products in impure extracts as interpretation of fragmentation patterns, coupled in some instances with knowledge of HPLC retention properties, can facilitate the partial identification of components when reference compounds are unavailable.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / methods
  • Ellagic Acid / analogs & derivatives
  • Ellagic Acid / analysis*
  • Fruit / chemistry*
  • Mass Spectrometry / methods
  • Plant Extracts / chemistry
  • Quercetin / analysis*
  • Quercetin / chemistry*
  • Rosaceae / chemistry*
  • Tannins / analysis*
  • Tannins / chemistry*


  • Plant Extracts
  • Tannins
  • Ellagic Acid
  • Quercetin