FISHing with locked nucleic acids (LNA): evaluation of different LNA/DNA mixmers

Mol Cell Probes. 2003 Aug;17(4):165-9. doi: 10.1016/s0890-8508(03)00048-3.

Abstract

Locked Nucleic Acids (LNA) constitute a novel class of DNA analogues that have an exceptionally high affinity towards complementary DNA and RNA. Using human classical satellite-2 repeat sequence clusters as targets, we demonstrate that LNA/DNA mixmers oligonucleotides are excellent probes for FISH combining high binding affinity with short hybridization time and even with the ability to hybridize without prior thermal denaturation of the template.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Probes*
  • DNA, Complementary
  • DNA, Satellite / analysis*
  • Genomics / methods
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Male
  • Nucleic Acid Conformation
  • Nucleic Acid Denaturation
  • Oligonucleotide Probes*
  • Oligonucleotides
  • Oligonucleotides, Antisense*
  • Temperature
  • Time Factors

Substances

  • DNA Probes
  • DNA, Complementary
  • DNA, Satellite
  • Oligonucleotide Probes
  • Oligonucleotides
  • Oligonucleotides, Antisense
  • locked nucleic acid