[Effect of low-power laser irradiation on the structure and properties of protein molecules under non-specific energy absorbance]

Fiziol Zh. 2003;49(2):96-9.
[Article in Ukrainian]

Abstract

The influence of the helium-neon laser emission on HSA was investigated at present work by means of the fluorescent probe MNBIS (4-morpholino-7-oxy-7-H-benzo-[de]-benzo-[4,5]-imidazo-[2,1-a]- izoquinolin-5-sulfonic acid) sensitive to protein conformational changes using. Fluorescence spectra parameters of the probe and kinetics of probe interaction with protein molecules were analyzed. Possible mechanisms of the helium-neon laser irradiation on water-protein solution structure are discussed.

Publication types

  • English Abstract

MeSH terms

  • Fluorescent Dyes / chemistry
  • Fluorescent Dyes / radiation effects
  • Helium
  • Humans
  • Hydrogen-Ion Concentration
  • Kinetics
  • Lasers*
  • Molecular Structure
  • Neon
  • Protein Binding
  • Protein Conformation
  • Proteins / chemistry*
  • Proteins / radiation effects*
  • Radiation Dosage
  • Serum Albumin / chemistry
  • Serum Albumin / radiation effects
  • Solutions / chemistry
  • Spectrometry, Fluorescence
  • Tryptophan / chemistry
  • Tyrosine / chemistry
  • Water / chemistry

Substances

  • Fluorescent Dyes
  • Proteins
  • Serum Albumin
  • Solutions
  • Water
  • Helium
  • Tyrosine
  • Neon
  • Tryptophan