Flow cytometric mapping of the leukotriene B4 receptor, BLT1, in human bone marrow and peripheral blood using specific monoclonal antibodies

Int Immunopharmacol. 2003 Oct;3(10-11):1467-75. doi: 10.1016/S1567-5769(03)00145-0.


We have previously raised two monoclonal antibodies (7B1, 14F11) recognizing the high-affinity leukotriene B4 receptor, BLT1. They were presently used to determine receptor surface expression in the hematopoietic system. In peripheral blood, BLT1 was primarily recognized in granulocytes, monocytes and, to a lower extent, in certain lymphocytes except the CD4 subpopulation. The expression pattern was similar in bone marrow cells. In vitro differentiation of CD34+ progenitor cells induced BLT1 expression within 7 days, which remained constant up to day 17 when a further increase was measured and maintained up to day 20. BLT1 expression was modified by inflammatory mediators: LPS, TNFalpha, fMLP, as well as LTB4 itself, caused a slight down-regulation at 30 min, an effect that was particularly marked with PMA, whereas the effect was least pronounced with IL-8. The antibodies have proved to be useful in an extensive mapping of BLT1 in both peripheral blood and bone marrow and as a tool to elucidate changes in the receptor expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antibodies, Monoclonal / immunology*
  • Bone Marrow Cells / immunology
  • Bone Marrow Cells / metabolism*
  • Flow Cytometry
  • Granulocytes / immunology
  • Granulocytes / metabolism*
  • Humans
  • Monocytes / immunology
  • Monocytes / metabolism*
  • Receptors, Leukotriene B4 / biosynthesis*
  • Receptors, Leukotriene B4 / immunology


  • Antibodies, Monoclonal
  • Receptors, Leukotriene B4