Epitope mapping and specificity of the anti-alpha-synuclein monoclonal antibody Syn-1 in mouse brain and cultured cell lines

Neurosci Lett. 2003 Oct 2;349(2):133-5. doi: 10.1016/s0304-3940(03)00781-x.


While alpha- and beta-synuclein largely overlap in their expression in the vertebrate brain, only alpha-synuclein accumulates in the fibrillar aggregates typical of Parkinson's disease. It is thus critical to have immunological reagents that distinguish between these two protein isoforms. The monoclonal antibody Syn-1 (Transduction Labs) has been frequently used for the specific detection of alpha-synuclein. In this report, the epitope for Syn-1 is localized within residues 91-99 of human alpha-synuclein. Sequence differences exist in this domain that account for the specificity of Syn-1 for alpha- versus beta-synuclein. However, Syn-1 also displays reactivity with additional species (approximately 45 kDa) in brain homogenates from both wild-type and alpha-synuclein null mice, indicating a potential for cross-reactivity with a protein species that is unrelated to alpha-synuclein in brain tissue or extracts.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies, Monoclonal*
  • Antibody Specificity*
  • Brain / immunology
  • Brain / metabolism*
  • Cells, Cultured
  • Epitope Mapping*
  • Humans
  • Immunoblotting
  • Mice
  • Mice, Knockout
  • Mutation
  • Nerve Tissue Proteins / genetics
  • Nerve Tissue Proteins / immunology*
  • Rats
  • Recombinant Proteins
  • Species Specificity
  • Synucleins
  • alpha-Synuclein
  • beta-Synuclein


  • Antibodies, Monoclonal
  • Nerve Tissue Proteins
  • Recombinant Proteins
  • SNCA protein, human
  • SNCB protein, human
  • Snca protein, mouse
  • Snca protein, rat
  • Sncb protein, mouse
  • Sncb protein, rat
  • Synucleins
  • alpha-Synuclein
  • beta-Synuclein