The effect of 3 types of fat: 1) Ghee, 2) Corn oil, 3) Subsidized vegetable oil (SVO) on serum and tissue lipids was studied by using adult albino male rats mean weight 114 g. Rats fed diet containing SVO had the highest serum cholesterol and LDL-cholesterol concentrations than those fed diet containing ghee or corn oil. Serum high density lipoprotein-cholesterol (HDL) concentration was highest in animals fed the ghee and lowest with those fed the SVO diet. On the other hand phospholipids values tend to be lower when feeding diets containing oils. Also serum triglycerides levels were higher on saturated fat diet than on the unsaturated fat diets. The same trends were found for liver cholesterol as in serum cholesterol. SVO diet gave the highest liver cholesterol concentration. Also SVO gave the highest heart phospholipids values.