The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of L-aspartate-derived amino acids and vitamins

J Biotechnol. 2003 Sep 4;104(1-3):5-25. doi: 10.1016/s0168-1656(03)00154-8.


The complete genomic sequence of Corynebacterium glutamicum ATCC 13032, well-known in industry for the production of amino acids, e.g. of L-glutamate and L-lysine was determined. The C. glutamicum genome was found to consist of a single circular chromosome comprising 3282708 base pairs. Several DNA regions of unusual composition were identified that were potentially acquired by horizontal gene transfer, e.g. a segment of DNA from C. diphtheriae and a prophage-containing region. After automated and manual annotation, 3002 protein-coding genes have been identified, and to 2489 of these, functions were assigned by homologies to known proteins. These analyses confirm the taxonomic position of C. glutamicum as related to Mycobacteria and show a broad metabolic diversity as expected for a bacterium living in the soil. As an example for biotechnological application the complete genome sequence was used to reconstruct the metabolic flow of carbon into a number of industrially important products derived from the amino acid L-aspartate.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Amino Acid Sequence
  • Amino Acids / biosynthesis*
  • Amino Acids / genetics
  • Aspartic Acid / genetics
  • Aspartic Acid / metabolism*
  • Base Sequence
  • Corynebacterium / classification
  • Corynebacterium / genetics*
  • Corynebacterium / metabolism*
  • Databases, Genetic
  • Gene Expression Profiling
  • Genome, Bacterial*
  • Molecular Sequence Data
  • Multienzyme Complexes / genetics
  • Multienzyme Complexes / metabolism
  • Proteome / genetics*
  • Proteome / metabolism*
  • Recombinant Proteins / biosynthesis
  • Sequence Analysis, DNA
  • Sequence Homology, Nucleic Acid
  • Vitamins / biosynthesis*
  • Vitamins / genetics


  • Amino Acids
  • Multienzyme Complexes
  • Proteome
  • Recombinant Proteins
  • Vitamins
  • Aspartic Acid