Fibroblast Cell Shape and Adhesion in Vitro Is Altered by Overexpression of the 7a and 7b Isoforms of Protocadherin 7, but Not the 7c Isoform

Cell Mol Biol Lett. 2003;8(3):735-41.

Abstract

Protocadherins (Pcdhs) are a family of cadherins considered to play an important role in the cell-cell adhesion of specific neurons in the central nervous system. Of the reported Pcdhs, relatively little is known about the functional role of protocadherin 7 (Pcdh7), and there is no evidence of Pcdh7 mediated cell-cell adhesion. To date, three splicing variants are known; they may have different effects on cell phenotype. We report here that mouse fibroblast L cells stably overexpressing the Pcdh7 isoforms 7a and 7b, but not 7c, showed a morphological change and Ca(2+)dependent cell adhesion.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Cadherins / genetics
  • Cadherins / metabolism*
  • Cell Adhesion
  • Cell Size
  • Fibroblasts / cytology
  • Fibroblasts / metabolism*
  • Immunohistochemistry
  • L Cells
  • Mice
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism

Substances

  • CDH7 protein, human
  • Cadherins
  • Protein Isoforms