Abstract
Pichia pastoris secretes few native proteins. However, the more than 1 g l(-1)of extracellularly expressed mannan interfered with the purification of our extracellularly expressed, non-glycosylated recombinant protein. Concanavalin A-agarose removed more than 95% of the unwanted mannan as monitored by phenol reaction. A(13)C-based NMR assay confirmed this improvement. Concanavalin A-agarose can assist the purification of extracellular expressed, non-glycosylated proteins from yeasts.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carbon Isotopes
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Chromatography, Ion Exchange
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Concanavalin A / metabolism*
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Electrophoresis, Polyacrylamide Gel
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Gene Expression Regulation, Fungal
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Magnetic Resonance Spectroscopy
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Mannans / isolation & purification*
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Pichia / metabolism*
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Plant Proteins / analysis
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Plant Proteins / metabolism
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Polysaccharides / analysis
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Recombinant Proteins / metabolism*
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Sepharose / metabolism*
Substances
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Carbon Isotopes
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Mannans
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Plant Proteins
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Polysaccharides
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Recombinant Proteins
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Concanavalin A
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Sepharose