Development of galanin-like immunoreactivity in the brain of the brown trout (Salmo trutta fario), with some observations on sexual dimorphism

Abstract

The development of galanin-like immunoreactive (GAL-ir) cells and fibers was investigated in the brain of brown trout embryos, alevins, juveniles, and adults (some spontaneously releasing their gametes). The earliest GAL-ir neurons appeared in the preoptic region and the primordial hypothalamic lobe of 12-mm embryos. After hatching, new GAL-ir neurons appeared in the lateral, anterior, and posterior tuberal nuclei, and in late alevins, GAL-ir neurons appeared in the area postrema. In juveniles, further GAL-ir populations appeared in the nucleus subglomerulosus and magnocellular preoptic nucleus. The GAL-ir neuronal groups present in juveniles were also observed in sexually mature adults, although the area postrema of males lacked immunoreactive neurons. Moreover, spawning males exhibited GAL-ir somata in the olfactory bulb and habenula, which were never observed in adult females or in developing stages. In adults, numerous GAL-ir fibers were observed in the ventral telencephalon, preoptic area, hypothalamus, neurohypophysis, mesencephalic tegmentum, ventral rhombencephalon, and area postrema. Moderate to low GAL-ir innervation was seen in the olfactory bulbs, dorsomedial telencephalon, epithalamus, medial thalamus, optic tectum, cerebellum, and rhombencephalic alar plate. There were large differences among regions in the GAL-ir innervation establishment time. In embryos, GAL-ir fibers appeared in the preoptic area and hypothalamus, indicating early expression of galanin in hypophysiotrophic centers. The presence of galanin immunoreactivity in the olfactory, reproductive, visual, and sensory-motor centers of the brain suggest that galanin is involved in many other brain functions. Furthermore, the distribution of GAL-ir elements observed throughout trout development indicates that galaninergic system maturation continues until sexual maturity.