Combination anti-inflammatory and antiviral therapy of influenza in a cotton rat model

Pediatr Pulmonol. 2003 Oct;36(4):290-4. doi: 10.1002/ppul.10320.


The cotton rat was evaluated as a model for anti-inflammatory and antiviral influenza therapy. Beginning 3 days after intranasal infection with 10(7) tissue culture infectious doses-50% (TCID)(50) of an H3N2 human influenza, animals were treated topically via intranasal lavage with a range of doses of triamcinolone acetonide (1, 4, or 16 mg/kg), alone or in combination with a neuraminidase inhibitor or anti-influenza convalescent serum. Pulmonary histopathologic changes were dramatically decreased in animals treated with 4 or 16 mg/kg of triamcinolone, with little additional benefit from addition of a neuraminidase inhibitor or topical serum, agents which were much less effective when used alone. A high degree of suppression of IFN-gamma levels was observed in all combinations where 4 or 16 mg/kg of triamcinolone were used. Viral replication was not prolonged by corticosteroid therapy. Tissue damage during influenza infection may be greatly reduced by combination antiviral and anti-inflammatory therapy.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Acetamides / therapeutic use*
  • Animals
  • Antiviral Agents / therapeutic use*
  • Disease Models, Animal
  • Drug Therapy, Combination
  • Glucocorticoids / therapeutic use*
  • Guanidines
  • Neuraminidase / antagonists & inhibitors
  • Orthomyxoviridae Infections / drug therapy*
  • Oseltamivir
  • Pyrans
  • Rats
  • Sialic Acids / therapeutic use*
  • Sigmodontinae
  • Triamcinolone Acetonide / administration & dosage
  • Triamcinolone Acetonide / therapeutic use*
  • Zanamivir


  • Acetamides
  • Antiviral Agents
  • Glucocorticoids
  • Guanidines
  • Pyrans
  • Sialic Acids
  • Oseltamivir
  • Neuraminidase
  • Triamcinolone Acetonide
  • Zanamivir