Scanning electron microscopy of intracellular organelles in the young odontoblasts of rats

Scanning Microsc. 1992 Dec;6(4):1089-95; discussion 1095-6.

Abstract

Intracellular structures of the odontoblasts were studied by scanning electron microscopy (SEM) using a modified AODO (aldehyde prefixed-osmium-DMSO-osmium) method. Well-developed flattened and layered rER (rough endoplasmic reticulum), paved with its associated ribosomes on its outer surface, were clearly observed in the odontoblast. Branched tubular mitochondria with nodules and swollen endings, interposing between and passing through the fenestrated layered rER, were demonstrated in the functional cells. Oblique and cross-sections of both the rER system and tubular mitochondria showed orthodox configurations similar to those usually described in transmission electron microscopy (TEM) studies. Many finger-like projections constructing the cristae directing towards the inner mitochondrial chamber were observed, and external chamber extending into the tubular cristae was also demonstrated.

MeSH terms

  • Animals
  • Dimethyl Sulfoxide
  • Endoplasmic Reticulum / ultrastructure*
  • Male
  • Microscopy, Electron, Scanning*
  • Mitochondria / ultrastructure*
  • Odontoblasts / ultrastructure*
  • Osmium Tetroxide
  • Rats
  • Rats, Wistar

Substances

  • Osmium Tetroxide
  • Dimethyl Sulfoxide