rDNA ITS sequence of Rhizopus oryzae: its application to classification and identification of lactic acid producers

Biosci Biotechnol Biochem. 2003 Aug;67(8):1725-31. doi: 10.1271/bbb.67.1725.


Rhizopus oryzae is an important organism for its production of organic acids such as lactic acid, fumaric acid, etc. To date, there were no easy methods to classify strains according to their acid production. The sequences of the ribosomal RNA-encoding DNA (rDNA) internal transcribed spacer (ITS) region of 64 strains of R. oryzae were analyzed and found to conserve mutations correspond to acid production. We have devised a way to use these mutations for a novel method to identify lactic-acid-producing Rhizopus oryzae, by designing specific polymerase chain reaction (PCR) primers on them. Touch down PCR using these primers amplified the ITS DNA of lactic acid producers specifically. By this method, we could isolate lactic acid producing strains from Indonesian fermented foods.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Biotechnology / methods
  • DNA, Fungal / genetics
  • DNA, Ribosomal Spacer / genetics*
  • Fermentation
  • Genes, Fungal / genetics
  • Lactic Acid / biosynthesis*
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction / methods
  • Rhizopus / classification*
  • Rhizopus / genetics*
  • Rhizopus / metabolism


  • DNA, Fungal
  • DNA, Ribosomal Spacer
  • Lactic Acid