Comparing protein abundance and mRNA expression levels on a genomic scale

Genome Biol. 2003;4(9):117. doi: 10.1186/gb-2003-4-9-117. Epub 2003 Aug 29.


Attempts to correlate protein abundance with mRNA expression levels have had variable success. We review the results of these comparisons, focusing on yeast. In the process, we survey experimental techniques for determining protein abundance, principally two-dimensional gel electrophoresis and mass-spectrometry. We also merge many of the available yeast protein-abundance datasets, using the resulting larger 'meta-dataset' to find correlations between protein and mRNA expression, both globally and within smaller categories.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Electrophoresis, Gel, Two-Dimensional
  • Gene Expression
  • Genomics / methods*
  • Humans
  • Mass Spectrometry
  • Proteins / genetics
  • Proteins / metabolism*
  • Proteomics / methods*
  • RNA, Messenger / genetics
  • RNA, Messenger / metabolism*


  • Proteins
  • RNA, Messenger