Monocyte chemoattractant protein-1 increases microglial infiltration and aggressiveness of gliomas

Ann Neurol. 2003 Sep;54(3):388-92. doi: 10.1002/ana.10679.


Macrophages are thought to represent a first line of defense in anti-tumor immunity. Despite infiltration by microglial cells, however, malignant gliomas are still highly aggressive tumors. We here identify monocyte chemoattractant protein-1 (MCP-1) as a critical chemoattractant for glioma-infiltrating microglial cells. MCP-1-transfected rat CNS-1 gliomas were massively infiltrated by microglial cells. Whereas MCP-1 did not promote the growth of CNS-1 cells in vitro, intracerebral CNS-1-transfected tumors grew more aggressively than control-transfected tumors. This provides the first functional evidence that MCP-1 recruits microglial cells to gliomas and promotes their growth in vivo. Microglial cells may support rather than suppress glioma growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chemokine CCL2 / physiology*
  • Chemotaxis
  • Corpus Striatum / transplantation
  • Culture Media, Conditioned / pharmacology
  • Female
  • Glioma / metabolism
  • Microglia / metabolism*
  • Neoplasm Invasiveness
  • Neoplasm Transplantation
  • Neurons / drug effects
  • Neurons / metabolism
  • Polymerase Chain Reaction
  • RNA, Messenger / analysis
  • Rats
  • Rats, Wistar
  • Transfection
  • Tumor Cells, Cultured


  • Chemokine CCL2
  • Culture Media, Conditioned
  • RNA, Messenger