Genetic and Cytological Characterization of the RecA-homologous Proteins Rad51 and Dmc1 of Schizosaccharomyces Pombe

Curr Genet. 2004 Jan;44(6):317-28. doi: 10.1007/s00294-003-0439-7. Epub 2003 Aug 29.

Abstract

The Schizosaccharomyces pombe rad51(+) and dmc1(+) genes code for homologues of the Escherichia coli recombination protein RecA. Deletion of rad51(+) causes slow growth, retardation of cell division and a decrease in viability. rad51Delta cells have a defect in mating-type switching. The DNA modification at the mating-type locus required for mating-type switching contributes to slow growth in the rad51 mutant. Cell mating is reduced in crosses homozygous for rad51Delta. Ectopic expression of the dmc1(+) gene allowed us to demonstrate that the reduction in meiotic recombination in dmc1 mutants is not caused by a disturbance of rad24 expression from the dmc1- rad24 bicistronic RNA. We describe the functional defects of terminally epitope-tagged Dmc1 and Rad51 and discuss it in terms of protein interaction. Presumptive Rad51 and Dmc1 foci were detected on spreads of meiotic chromatin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenosine Triphosphatases*
  • Animals
  • Antibodies, Monoclonal
  • Cell Compartmentation
  • Cell Cycle Proteins / biosynthesis*
  • Cell Cycle Proteins / genetics
  • Crossing Over, Genetic
  • DNA-Binding Proteins / biosynthesis*
  • DNA-Binding Proteins / genetics
  • Epitopes
  • Escherichia coli
  • Gene Deletion
  • Gene Expression Regulation, Fungal
  • Genetic Vectors
  • Green Fluorescent Proteins
  • Immunochemistry
  • Luminescent Proteins / biosynthesis
  • Luminescent Proteins / genetics
  • Mice
  • Mice, Knockout
  • Nuclear Proteins
  • Phosphate-Binding Proteins
  • Rad51 Recombinase
  • Rec A Recombinases / genetics*
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / genetics
  • Schizosaccharomyces / genetics
  • Schizosaccharomyces / metabolism*
  • Schizosaccharomyces / ultrastructure
  • Schizosaccharomyces pombe Proteins*
  • Sequence Homology, Nucleic Acid

Substances

  • Antibodies, Monoclonal
  • Cell Cycle Proteins
  • DNA-Binding Proteins
  • Dmc1 protein, mouse
  • Epitopes
  • Luminescent Proteins
  • Nuclear Proteins
  • Phosphate-Binding Proteins
  • RHP51 protein, S pombe
  • Recombinant Fusion Proteins
  • Schizosaccharomyces pombe Proteins
  • Green Fluorescent Proteins
  • Rad51 Recombinase
  • Rad51 protein, mouse
  • Rec A Recombinases
  • Adenosine Triphosphatases