OsBP-73, a rice gene, encodes a novel DNA-binding protein with a SAP-like domain and its genetic interference by double-stranded RNA inhibits rice growth

Plant Mol Biol. 2003 Jun;52(3):579-90. doi: 10.1023/a:1024854101965.

Abstract

The SAP domain is a recently defined DNA binding domain that forms a helix-extended-helix structure. SAP proteins have been implicated in nuclear architecture and/or RNA metabolism. In this paper, we describe the cloning and characterization of a rice gene, OsBP-73, encoding a 375 amino acid protein with a SAP-like domain. We identified the binding sequence of OsBP-73 by gel retardation assays and southwestern blotting. Northern blot analysis demonstrated that OsBP-73 is weakly expressed in root, leaf and immature seed. OsBP-73 gene expression was also examined by histochemical studies of transgenic rice plants carrying an OsBP-73 5'/GUS reporter gene. The reporter gene is mainly expressed in the tissues with high cell division activities, such as root tip, stem node, panicle and immature seed. Genetic interference of OsBP-73 gene expression by double-stranded RNA strikingly inhibits the whole plant growth but does not affect the passage from the juvenile to adult phase. These results suggest that OsBP-73 may play an important role in the regulation of cell proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Binding Sites / genetics
  • Blotting, Northern
  • DNA, Complementary / chemistry
  • DNA, Complementary / genetics
  • DNA, Complementary / isolation & purification
  • DNA, Plant / chemistry
  • DNA, Plant / genetics
  • DNA, Plant / isolation & purification
  • DNA-Binding Proteins / genetics*
  • DNA-Binding Proteins / metabolism
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Plant
  • Genes, Plant / genetics*
  • Molecular Sequence Data
  • Oligonucleotides / metabolism
  • Oryza / genetics*
  • Oryza / growth & development
  • Plants, Genetically Modified
  • Protein Binding
  • RNA Interference / physiology*
  • RNA, Double-Stranded / genetics
  • RNA, Plant / genetics
  • RNA, Plant / metabolism
  • Recombinant Proteins / metabolism
  • Sequence Analysis, DNA
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • DNA, Plant
  • DNA-Binding Proteins
  • Oligonucleotides
  • RNA, Double-Stranded
  • RNA, Plant
  • Recombinant Proteins

Associated data

  • GENBANK/AJ315790
  • GENBANK/AJ315791