Use of multiplex PCR to identify Staphylococcus aureus adhesins involved in human hematogenous infections

J Clin Microbiol. 2003 Sep;41(9):4465-7. doi: 10.1128/JCM.41.9.4465-4467.2003.

Abstract

We have developed a multiplex PCR procedure to determine the distribution of nine adhesin genes in Staphylococcus aureus isolates. Only genes encoding bone sialoprotein binding protein and fibronectin binding protein B were significantly associated with hematogenous osteomyelitis/arthritis and native-valve endocarditis, respectively, suggesting their involvement in hematogenous tissue infections.

MeSH terms

  • Adhesins, Bacterial / genetics*
  • Adhesins, Bacterial / physiology
  • Arthritis, Infectious / microbiology*
  • Bacterial Proteins / genetics
  • Carrier Proteins / genetics
  • Coagulase / genetics
  • Endocarditis, Bacterial / microbiology*
  • Humans
  • Nose / microbiology
  • Osteomyelitis / microbiology*
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity

Substances

  • Adhesins, Bacterial
  • Bacterial Proteins
  • Carrier Proteins
  • Coagulase
  • adhesin, Staphylococcus aureus
  • fibronectin-binding proteins, bacterial