Nuclear membrane proteins with potential disease links found by subtractive proteomics

Science. 2003 Sep 5;301(5638):1380-2. doi: 10.1126/science.1088176.


To comprehensively identify integral membrane proteins of the nuclear envelope (NE), we prepared separately NEs and organelles known to cofractionate with them from liver. Proteins detected by multidimensional protein identification technology in the cofractionating organelles were subtracted from the NE data set. In addition to all 13 known NE integral proteins, 67 uncharacterized open reading frames with predicted membrane-spanning regions were identified. All of the eight proteins tested targeted to the NE, indicating that there are substantially more integral proteins of the NE than previously thought. Furthermore, 23 of these mapped within chromosome regions linked to a variety of dystrophies.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Algorithms
  • Animals
  • Cell Fractionation
  • Chromosome Mapping
  • DNA, Complementary
  • Genetic Diseases, Inborn / genetics*
  • Genetic Linkage
  • Humans
  • Liver / chemistry
  • Liver / ultrastructure
  • Membrane Proteins / analysis*
  • Membrane Proteins / genetics
  • Membrane Proteins / isolation & purification
  • Mice
  • Mice, Inbred Strains
  • Nuclear Envelope / chemistry*
  • Nuclear Proteins / analysis*
  • Nuclear Proteins / genetics
  • Nuclear Proteins / isolation & purification
  • Open Reading Frames
  • Protein Structure, Tertiary
  • Proteomics*
  • Rats
  • Rats, Sprague-Dawley


  • DNA, Complementary
  • Membrane Proteins
  • Nuclear Proteins