Isolation and characterization of viruses related to the SARS coronavirus from animals in southern China

Science. 2003 Oct 10;302(5643):276-8. doi: 10.1126/science.1087139. Epub 2003 Sep 4.

Abstract

A novel coronavirus (SCoV) is the etiological agent of severe acute respiratory syndrome (SARS). SCoV-like viruses were isolated from Himalayan palm civets found in a live-animal market in Guangdong, China. Evidence of virus infection was also detected in other animals (including a raccoon dog, Nyctereutes procyonoides) and in humans working at the same market. All the animal isolates retain a 29-nucleotide sequence that is not found in most human isolates. The detection of SCoV-like viruses in small, live wild mammals in a retail market indicates a route of interspecies transmission, although the natural reservoir is not known.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Animals, Wild / virology*
  • Antibodies, Viral / blood
  • Blotting, Western
  • Carnivora / virology*
  • China
  • Coronavirus / classification
  • Coronavirus / genetics
  • Coronavirus / immunology
  • Coronavirus / isolation & purification*
  • Coronavirus Infections / veterinary
  • Coronavirus Infections / virology
  • Disease Reservoirs
  • Feces / virology
  • Genome, Viral
  • Humans
  • Membrane Glycoproteins / chemistry
  • Membrane Glycoproteins / genetics
  • Molecular Sequence Data
  • Neutralization Tests
  • Nose / virology
  • Open Reading Frames / genetics
  • Phylogeny
  • Polymorphism, Genetic
  • Reverse Transcriptase Polymerase Chain Reaction
  • SARS Virus / classification
  • SARS Virus / genetics
  • SARS Virus / immunology
  • SARS Virus / isolation & purification*
  • Sequence Deletion
  • Sequence Homology, Nucleic Acid
  • Spike Glycoprotein, Coronavirus
  • Viral Envelope Proteins / chemistry
  • Viral Envelope Proteins / genetics
  • Viral Proteins / chemistry
  • Viral Proteins / genetics

Substances

  • Antibodies, Viral
  • Membrane Glycoproteins
  • Spike Glycoprotein, Coronavirus
  • Viral Envelope Proteins
  • Viral Proteins
  • spike glycoprotein, SARS-CoV

Associated data

  • GENBANK/AY304486
  • GENBANK/AY304487
  • GENBANK/AY304488
  • GENBANK/AY304489