The applications of biosensors and sensing technology in environment analysis and monitoring in water, air and others are reviewed, and the prospects of biosensor and sensing technology are discussed in this paper, including the determinations of BOD, phenols, nitrate and organophosphorous compounds, and the monitoring of red tides in water, the detections of carbon dioxide, sulfur dioxide and nitrogen oxide in atmosphere; the detections of poisonous residue and persistent organic pollutants; toxicity assessment and the determination of bacteria population.