Functional CD5+ B cells develop predominantly in the spleen of NOD/SCID/gammac(null) (NOG) mice transplanted either with human umbilical cord blood, bone marrow, or mobilized peripheral blood CD34+ cells

Exp Hematol. 2003 Sep;31(9):789-97. doi: 10.1016/s0301-472x(03)00193-0.


Objective: Human CD5+ B cells are the major B cell subset in fetal spleen and umbilical cord blood (CB), and their number gradually diminishes in both spleen and peripheral blood from infancy through childhood while conventional B cells increase. In this study, we investigated whether CD5+ cells differentiate from adult hematopoietic stem cells (HSCs) as well as fetal ones in immunodeficient mice.

Methods: In our system, NOD/SCID/gammac(null) (NOG) mice were transplanted with CD34+ cells from CB (hCB model), adult bone marrow (hBM model), and mobilized peripheral blood (hMPB model).

Results: In these model mice, a high proportion of CD19+IgM+CD5+ mature B cells appeared in the spleen, regardless of the CD34+ cell origin, 4 to 8 weeks after transplantation, while the majority were CD19+IgM-CD5- immature B cells in BM. The CD19+CD5- BM cells showed to express CD5 after the coculture with NOG spleen cells. In the sera of immunized hCB model mice with DNP-KLH, antigen-specific IgM but not IgG was enhanced.

Conclusion: Our results show that adult CD34+ cells develop into functional CD5+ B cells in NOG spleen as much as fetal CD34+ cells do.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, CD34 / immunology
  • B-Lymphocytes / cytology
  • B-Lymphocytes / immunology*
  • Bone Marrow Cells / cytology
  • Bone Marrow Cells / immunology
  • CD5 Antigens / immunology
  • Cell Differentiation / immunology*
  • Cell Lineage / immunology
  • Fetal Blood / cytology
  • Fetal Blood / immunology
  • Mice
  • Mice, Inbred NOD
  • Mice, SCID
  • Spleen / cytology
  • Spleen / immunology*
  • Stem Cell Transplantation*
  • Transplantation, Heterologous


  • Antigens, CD34
  • CD5 Antigens