Community-acquired Methicillin-Resistant Staphylococcus Aureus Carrying Panton-Valentine Leukocidin Genes: Worldwide Emergence

Emerg Infect Dis. 2003 Aug;9(8):978-84. doi: 10.3201/eid0908.030089.

Abstract

Infections caused by community-acquired (CA)-methicillin--resistant Staphylococcus aureus (MRSA) have been reported worldwide. We assessed whether any common genetic markers existed among 117 CA-MRSA isolates from the United States, France, Switzerland, Australia, New Zealand, and Western Samoa by performing polymerase chain reaction for 24 virulence factors and the methicillin-resistance determinant. The genetic background of the strain was analyzed by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). The CA-MRSA strains shared a type IV SCCmec cassette and the Panton-Valentine leukocidin locus, whereas the distribution of the other toxin genes was quite specific to the strains from each continent. PFGE and MLST analysis indicated distinct genetic backgrounds associated with each geographic origin, although predominantly restricted to the agr3 background. Within each continent, the genetic background of CA-MRSA strains did not correspond to that of the hospital-acquired MRSA.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Toxins
  • Communicable Diseases, Emerging / epidemiology
  • Communicable Diseases, Emerging / genetics*
  • Community-Acquired Infections / epidemiology
  • Community-Acquired Infections / genetics*
  • Electrophoresis, Gel, Pulsed-Field
  • Exotoxins
  • Humans
  • Leukocidins / genetics*
  • Methicillin Resistance / genetics*
  • Microbial Sensitivity Tests
  • Staphylococcus aureus* / drug effects
  • Staphylococcus aureus* / genetics
  • Staphylococcus aureus* / isolation & purification

Substances

  • Bacterial Toxins
  • Exotoxins
  • Leukocidins
  • Panton-Valentine leukocidin