Multiprotein signaling complexes and regulation of cardiac phenotype

J Mol Cell Cardiol. 2003 Sep;35(9):1027-33. doi: 10.1016/s0022-2828(03)00175-5.


There is increasing evidence across disciplines for multiprotein complexes as a mechanism for signal transduction in various cell types. These multiprotein complexes appear to be altered as a direct mechanism to confer signaling responses and to alter phenotype. Although classical experimental techniques are effective to delineate the signaling role of one or a few molecules, they are limited in their ability to thoroughly characterize multiprotein complexes. In this review, the contribution of functional proteomics to a protein complex-based portrait of cardiac cell signaling will be examined along with a discussion and brief evaluation of the state-of-the-art proteomic approaches for multiprotein complex analysis.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Biomarkers / analysis
  • Electrophoresis, Gel, Two-Dimensional
  • Humans
  • Macromolecular Substances
  • Models, Biological
  • Multiprotein Complexes
  • Myocardium / cytology
  • Myocardium / metabolism*
  • Myocytes, Cardiac / metabolism
  • Phenotype
  • Protein Kinase C / analysis
  • Protein Kinase C-epsilon
  • Proteome / analysis
  • Proteomics
  • Signal Transduction*
  • src-Family Kinases / metabolism


  • Biomarkers
  • Macromolecular Substances
  • Multiprotein Complexes
  • Proteome
  • src-Family Kinases
  • PRKCE protein, human
  • Protein Kinase C
  • Protein Kinase C-epsilon