Differential regulation of TNF-alpha, IL-6 and IL-10 in UVB-irradiated human keratinocytes via cyclic AMP/protein kinase A pathway

Cytokine. 2003 Aug 21-Sep 7;23(4-5):138-49. doi: 10.1016/s1043-4666(03)00224-2.

Abstract

Pro-inflammatory cytokines are important mediators of cutaneous cellular activities during many skin diseases. In the present study, we investigated the production of tumor necrosis factor-alpha (TNF-alpha), IL-6 and IL-10 by UVB-irradiated human keratinocytes NCTC 2544 cell line in the presence of cAMP-elevating agents and we attempted to determine the implication of cyclic AMP/PKA pathway in the regulation of cytokine gene expression. Cytokine mRNA expression levels and cytokine concentrations were investigated by reverse transcription polymerase chain reaction and by ELISA method, respectively. Treatment of UVB-irradiated NCTC 2544 cells with drugs known to enhance cAMP concentration [dibutyryl cAMP, PGE(2) and cholera toxin] results in a significant decrease of TNF-alpha mRNA expression whereas IL-6 and IL-10 mRNAs were enhanced. In the same experimental conditions, treatment of irradiated keratinocytes with PKA inhibitors [H89 and PKA inhibitor (PKAi)] induced a significant inhibition of mRNA expression for all tested cytokines. Except for IL-10, the pharmacological effect of cAMP-elevating agents or PKA inhibitors on radiation-induced TNF-alpha and IL-6 mRNA expression was associated with a concomitant regulation of cytokine release. Taken together our results showed: (i) a differential regulation of TNF-alpha, IL-6 and IL-10 in UVB-irradiated human keratinocytes via cyclic AMP/protein kinase A pathway, and (ii) a possible reduction of deleterious inflammatory effects of cytokine following UVB-irradiation by using pharmacological agents that regulate both the intracellular cAMP levels and the cellular PKA activity.

MeSH terms

  • Blotting, Northern
  • Bucladesine / pharmacology
  • Carrier Proteins / pharmacology
  • Cell Line
  • Cholera Toxin / pharmacology
  • Cyclic AMP / physiology*
  • Cyclic AMP-Dependent Protein Kinases / physiology*
  • Cytokines / genetics*
  • Dinoprostone / pharmacology
  • Down-Regulation
  • Enzyme-Linked Immunosorbent Assay
  • Gene Expression Regulation / drug effects
  • Gene Expression Regulation / radiation effects
  • Humans
  • Interleukin-10 / genetics
  • Interleukin-6 / genetics
  • Intracellular Signaling Peptides and Proteins*
  • Isoquinolines / pharmacology
  • Keratinocytes / drug effects
  • Keratinocytes / metabolism
  • Keratinocytes / radiation effects*
  • Peptide Fragments / pharmacology
  • RNA, Messenger / drug effects
  • RNA, Messenger / metabolism
  • RNA, Messenger / radiation effects
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sulfonamides*
  • Tumor Necrosis Factor-alpha / genetics
  • Ultraviolet Rays
  • Up-Regulation

Substances

  • Carrier Proteins
  • Cytokines
  • Interleukin-6
  • Intracellular Signaling Peptides and Proteins
  • Isoquinolines
  • Peptide Fragments
  • RNA, Messenger
  • Sulfonamides
  • Tumor Necrosis Factor-alpha
  • protein kinase inhibitor (6-22)-amide
  • Interleukin-10
  • Bucladesine
  • Cholera Toxin
  • Cyclic AMP
  • Cyclic AMP-Dependent Protein Kinases
  • Dinoprostone
  • N-(2-(4-bromocinnamylamino)ethyl)-5-isoquinolinesulfonamide