Synergies of microtissue design, viral transduction and adjustable transgene expression for regenerative medicine

Biotechnol Appl Biochem. 2004 Feb;39(Pt 1):3-16. doi: 10.1042/BA20030124.


In the past decade, regenerative medicine has evolved as an interdisciplinary field, integrating expertise from the medical, life- and material-science communities. Recent advances in tissue engineering, gene therapy, gene-function analysis, animal-free drug testing, drug discovery, biopharmaceutical manufacturing and cell-phenotype engineering have capitalized on a core technology portfolio including artificial microtissue design, viral transduction and precise transcription dosing of therapeutic or phenotype-modulating transgenes. We provide a detailed overview on recent progress in these core technologies and comment on their synergistic impact on current and future human therapies.

Publication types

  • Review

MeSH terms

  • Animals
  • Gene Expression Regulation
  • Gene Transfer Techniques*
  • Genetic Therapy
  • Genetic Vectors / genetics
  • Humans
  • Regenerative Medicine / methods*
  • Tissue Engineering / methods*
  • Transduction, Genetic / methods
  • Transgenes / genetics
  • Viruses / genetics