A one-step microbial DNA extraction method using "Chelex 100" suitable for gene amplification

Res Microbiol. 1992 Oct;143(8):785-90. doi: 10.1016/0923-2508(92)90107-y.


"Chelex 100" chelating resin has been previously proposed for the rapid extraction of human DNA for polymerase chain reaction. Protocols are given for the rapid extraction of bacterial and viral DNA from cultures or clinical samples. The DNA samples obtained were suitable for use in polymerase chain reaction.

MeSH terms

  • Chelating Agents*
  • DNA, Bacterial / isolation & purification*
  • Gene Amplification
  • Gram-Negative Bacteria / genetics*
  • Gram-Positive Bacteria / genetics*
  • In Vitro Techniques
  • Polymerase Chain Reaction / methods*
  • Resins, Synthetic
  • Streptococcus agalactiae / genetics


  • Chelating Agents
  • DNA, Bacterial
  • Resins, Synthetic
  • Chelex 100