Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 43 (1-4), 409-19

The Respiratory Central Pattern Generator (CPG) of Lymnaea Reconstructed in Vitro

Affiliations
  • PMID: 1299129

The Respiratory Central Pattern Generator (CPG) of Lymnaea Reconstructed in Vitro

N I Syed et al. Acta Biol Hung.

Abstract

We have recently developed a model system for testing the necessity, appropriateness and sufficiency of individual components of the respiratory central pattern generator (CPG) in Lymnaea stagnalis. In order to examine the intrinsic and network properties of the three CPG interneurons (R.Pe.D1, V.D4 and Ip.3.I), these cells were isolated and cultured in vitro. All of these cells exhibited extensive neurite outgrowth within 18-24 h of plating in conditioned medium. These isolated neurons maintained their intrinsic properties in culture and also formed specific synapses with each other similar to those observed in vivo. However, only when all three interneurons were plated together was it possible to initiate the alternating rhythm characteristic of the respiratory activity observed in semi-intact or isolated brain preparations.

Similar articles

See all similar articles

Cited by 5 PubMed Central articles

LinkOut - more resources

Feedback