Rapid generation of region specific probes by chromosome microdissection and their application

Nat Genet. 1992 Apr;1(1):24-8. doi: 10.1038/ng0492-24.


The strategy presented here to identify unequivocally cryptic chromosomal rearrangements has relevance to both prenatal and postnatal cytogenetic analysis as well as the analysis of tumour-associated chromosome rearrangements. Microdissection and in vitro amplification of specific chromosomal regions are performed, followed by labelling for fluorescent in situ hybridization (FISH) to normal metaphase chromosomes (Micro-FISH). Micro-FISH probes have been used successfully to determine the derivation of chromosome segments unidentifiable by standard chromosome banding analysis. Micro-FISH probes (created in less than 24 hours) now make it possible to identify explicitly the chromosome constitution of virtually all cytologically visible chromosome rearrangements.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • Chromosome Aberrations
  • Chromosome Deletion
  • Chromosomes, Human / ultrastructure*
  • DNA / genetics
  • DNA Probes
  • Female
  • Gene Rearrangement
  • Humans
  • In Situ Hybridization, Fluorescence / methods*
  • Molecular Probe Techniques*
  • Molecular Sequence Data
  • Neoplasms / genetics
  • Polymerase Chain Reaction
  • Pregnancy
  • Prenatal Diagnosis
  • Translocation, Genetic


  • DNA Probes
  • DNA