Rescue of erythroid development in gene targeted GATA-1- mouse embryonic stem cells

Nat Genet. 1992 May;1(2):92-8. doi: 10.1038/ng0592-92.


Development of definitive (fetal liver-derived) red cells is blocked by a targeted mutation in the gene encoding the transcription factor GATA-1. We used in vitro differentiation of GATA-1- mouse embryonic stem (ES) cells to reveal a requirement for GATA-1 during primitive (yolk sac-derived) erythropoiesis and to establish a rescue assay. We show that the block to development includes primitive, as well as definitive, erythroid cells and is complete at the level of globin RNA expression; that the introduction of a normal GATA-1 gene restores developmental potential both in vivo and in vitro; and that efficient rescue is dependent on a putative autoregulatory GATA-motif in the distal promoter. Use of in vitro differentiated ES cells bridges a gap between conventional approaches to gene function in cell lines and analysis of loss of function mutations in the whole animal.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Cell Differentiation / genetics
  • Cells, Cultured
  • DNA-Binding Proteins / genetics*
  • Embryo, Mammalian / cytology
  • Erythroid-Specific DNA-Binding Factors
  • Erythropoiesis / genetics*
  • GATA1 Transcription Factor
  • Humans
  • Mice
  • Mutation
  • Stem Cells / cytology*
  • Transcription Factors / genetics*
  • Transfection


  • DNA-Binding Proteins
  • Erythroid-Specific DNA-Binding Factors
  • GATA1 Transcription Factor
  • GATA1 protein, human
  • Gata1 protein, mouse
  • Transcription Factors