Characterization of a pollen-specific cDNA from sunflower encoding a zinc finger protein

Plant J. 1992 Sep;2(5):713-21.


We have isolated, via differential hybridization screening of a floral cDNA library from sunflower, a cDNA clone that hybridizes to a 1100 nucleotide-long mRNA found exclusively in mature pollen grains. The cDNA encodes a 219 amino acid-long polypeptide containing two potential zinc fingers alternating with two basic domains. A similar organization is found in the erythroid-specific transcription factor Eryf1 from chicken and its murine homolog GF-1. The C-terminus of the protein contains a sixfold repeat of the pentapeptide sequence (S,T,A)(E,D)TQN. These features suggest that the SF3 protein is a transcription factor required for the expression of late pollen genes. The SF3 gene is a member of a multicopy gene family. A genomic copy of the gene has been isolated and sequenced; it is split by four short, AT-rich introns.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Biological Evolution
  • Cloning, Molecular
  • DNA-Binding Proteins / genetics
  • Erythroid-Specific DNA-Binding Factors
  • Helianthus / genetics*
  • Introns / genetics
  • Molecular Sequence Data
  • Multigene Family / genetics
  • Plant Proteins / genetics*
  • Pollen
  • Repetitive Sequences, Nucleic Acid / genetics
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Transcription Factors / genetics*
  • Transcription, Genetic
  • Zinc Fingers / genetics*


  • DNA-Binding Proteins
  • Erythroid-Specific DNA-Binding Factors
  • Plant Proteins
  • SF3 protein, Helianthus annuus
  • Transcription Factors

Associated data

  • GENBANK/X64392