Efficient methods for production of monoclonal antibodies for forensic applications

Nihon Hoigaku Zasshi. 1992 Dec;46(6):424-7.

Abstract

To simplify the labor-intensive process of making hybridomas, we fused popliteal lymph node cells, used an autoclavable serum-free culture medium throughout, and cloned hybridomas with human blood cell feeders. To make the best possible use of the serum-free medium, we adapted mouse myeloma cells to it. Using as little as 0.2 ml of human blood per culture plate, we successfully cloned hybridomas and established a hybrid cell line producing anti-peroxidase antibody. Our protocol affords a considerable saving on time, labor, and cost, and hopefully will encourage the forensic scientist to undertake the production of monoclonal antibodies.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis*
  • Cloning, Molecular
  • Culture Media
  • Forensic Medicine*
  • Humans
  • Hybridomas
  • Mice

Substances

  • Antibodies, Monoclonal
  • Culture Media