Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs

Plant J. 1992 May;2(3):417-22. doi: 10.1111/j.1365-313x.1992.00417.x.

Abstract

An Arabidopsis thaliana cDNA bank has been constituted in a Saccharomyces cerevisiae expression vector based on the phosphoglycerate kinase (PGK) promoter and terminator. This bank was used to complement eight S. cerevisiae auxotrophic markers. All of them were corrected. These results confirm the quality of the bank and the feasibility of cloning plant genes by yeast mutant complementation. The cDNA complementing the ura1 yeast mutant was sequenced, analysed and shown to encode a dihydroorotic (DHO) dehydrogenase sequence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics
  • Base Sequence
  • Cloning, Molecular
  • DNA / genetics*
  • Gene Library
  • Genetic Complementation Test
  • Genetic Vectors
  • Molecular Sequence Data
  • Mutation
  • Oxidoreductases / genetics
  • Oxidoreductases Acting on CH-CH Group Donors*
  • Plants / genetics*
  • Saccharomyces cerevisiae / genetics*

Substances

  • DNA
  • Oxidoreductases
  • Oxidoreductases Acting on CH-CH Group Donors
  • dihydroorotate dehydrogenase

Associated data

  • GENBANK/F32729
  • GENBANK/X62909