Plasma beta-carotene response in humans after meals supplemented with dietary pectin

Am J Clin Nutr. 1992 Jan;55(1):96-9. doi: 10.1093/ajcn/55.1.96.


The purpose of this study was to determine the effect of pectin on plasma response to beta-carotene in humans. Using a crossover design, we evaluated the effect on plasma beta-carotene in seven subjects when 12 g citrus pectin was added to a 2092 kJ (500 kcal) controlled meal with 25 mg beta-carotene. Plasma samples were collected at 0, 8, 30, 48, and 192 h after the meals. Plasma beta-carotene was quantified with the use of HPLC. The increase in plasma beta-carotene concentration was significantly reduced by pectin at 30 and 192 h (paired t test; P less than 0.005 and less than 0.05, respectively). Mean percent increase in plasma beta-carotene concentration at 30 h after the meal with beta-carotene was reduced by more than one-half when pectin was added to the meal. These results indicate that the inhibitory effect of pectin may provide one explanation for observations of reduced plasma beta-carotene response in humans after the ingestion of carotenoid-rich foods when compared with equivalent doses of beta-carotene supplements.

Publication types

  • Clinical Trial
  • Controlled Clinical Trial
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Body Mass Index
  • Carotenoids / administration & dosage
  • Carotenoids / blood*
  • Cholesterol / blood
  • Cholesterol, HDL / blood
  • Dietary Fiber / administration & dosage*
  • Female
  • Humans
  • Pectins / administration & dosage*
  • Random Allocation
  • beta Carotene


  • Cholesterol, HDL
  • Dietary Fiber
  • beta Carotene
  • Carotenoids
  • Pectins
  • Cholesterol