Abstract
Three cDNA clones, mGluR2, mGluR3, and mGluR4, were isolated from a rat brain cDNA library by cross-hybridization with the cDNA for a metabotropic glutamate receptor (mGluR1). The cloned receptors show considerable sequence similarity with mGluR1 and possess a large extracellular domain preceding the seven putative membrane-spanning segments. mGluR2 is expressed in some particular neuronal cells different from those expressing mGluR1 and mediates an efficient inhibition of forskolin-stimulated cAMP formation in cDNA-transfected cells. The mGluRs thus form a novel family of G protein-coupled receptors that differ in their signal transduction and expression patterns.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Brain Chemistry
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CHO Cells
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Cell Membrane / chemistry
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Cerebellum / chemistry
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Cerebral Cortex / chemistry
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Cloning, Molecular
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Colforsin / pharmacology
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Cricetinae
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Cyclic AMP / biosynthesis
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DNA / chemistry
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DNA / isolation & purification
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Gene Expression
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Molecular Sequence Data
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Nucleic Acid Hybridization
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Olfactory Bulb / chemistry
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RNA, Messenger / analysis
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Rats
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Receptors, Glutamate
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Receptors, Neurotransmitter / chemistry
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Receptors, Neurotransmitter / genetics*
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Signal Transduction
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Tissue Distribution
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Transfection
Substances
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RNA, Messenger
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Receptors, Glutamate
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Receptors, Neurotransmitter
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Colforsin
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DNA
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Cyclic AMP