Mapping T-cell receptor-peptide contacts by variant peptide immunization of single-chain transgenics

Nature. 1992 Jan 16;355(6357):224-30. doi: 10.1038/355224a0.


To test models of T-cell recognition, mice transgenic for T-cell receptor alpha or beta chain have been immunized with variant peptides that force changes in the resulting T-cell response. In particular, charge substitutions on the peptide often elicit reciprocal charges in the junctional (CDR3) sequences of T-cell receptor V alpha or V beta chains, indicating direct T-cell receptor-peptide contact, and allowing derivation of a topology for the T-cell receptor-MHC interaction. At one position on the peptide, variants transformed a homogeneous V beta response into a very heterogeneous one.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Antigens, Differentiation, T-Lymphocyte / genetics
  • Antigens, Differentiation, T-Lymphocyte / immunology
  • Base Sequence
  • Binding, Competitive
  • CD3 Complex
  • Columbidae
  • Cytochrome c Group / immunology*
  • Cytochrome c Group / metabolism
  • Genetic Variation*
  • Kinetics
  • Lymph Nodes / immunology
  • Lymphocyte Activation
  • Lymphocytes / immunology
  • Macromolecular Substances
  • Major Histocompatibility Complex
  • Mice
  • Mice, Transgenic
  • Models, Structural
  • Molecular Sequence Data
  • Moths
  • Oligodeoxyribonucleotides
  • Protein Conformation
  • Receptors, Antigen, T-Cell / genetics
  • Receptors, Antigen, T-Cell / immunology
  • Receptors, Antigen, T-Cell / metabolism*


  • Antigens, Differentiation, T-Lymphocyte
  • CD3 Complex
  • Cytochrome c Group
  • Macromolecular Substances
  • Oligodeoxyribonucleotides
  • Receptors, Antigen, T-Cell