9-cis retinoic acid stereoisomer binds and activates the nuclear receptor RXR alpha

Nature. 1992 Jan 23;355(6358):359-61. doi: 10.1038/355359a0.


Vitamin A (retinol) and its natural derivatives are required for many physiological processes. The activity of retinoids is thought to be mediated by interactions with two subfamilies of nuclear retinoic acid receptors, RAR and RXR. The RARs bind all-trans retinoic acid (t-RA) with high affinity and alter gene expression as a consequence of this direct ligand interaction. RXR alpha is activated by t-RA, yet has little binding affinity for this ligand. t-RA may be converted to a more proximate ligand that directly binds and activates RXR alpha, and we have developed a method of nuclear receptor-dependent ligand trapping to test this hypothesis. Here we report the identification of a stereoisomer of retinoic acid, 9-cis retinoic acid, which directly binds and activates RXR alpha. These results suggest a new role for isomerization in the physiology of natural retinoids.

MeSH terms

  • Animals
  • Base Sequence
  • Binding, Competitive
  • Carrier Proteins / genetics
  • Carrier Proteins / isolation & purification
  • Carrier Proteins / metabolism*
  • Cell Line
  • Cell Nucleus / metabolism*
  • Chromatography, High Pressure Liquid
  • Humans
  • Kinetics
  • Liver / metabolism
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Polymerase Chain Reaction / methods
  • Protein Binding
  • RNA, Messenger / genetics
  • Receptors, Retinoic Acid
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / metabolism
  • Stereoisomerism
  • Transcription, Genetic
  • Transfection
  • Tretinoin / metabolism*


  • Carrier Proteins
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Receptors, Retinoic Acid
  • Recombinant Proteins
  • Tretinoin